Background:

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen P2 containment. Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.

Species:

Homo sapiens, human

Tissue:

Mammary gland, breast; derived from metastatic site: pleural effusion

Disease:

Breast adenocarcinoma

Gender:

Female, 69 years

Morphology:

Epithelial-like cells growing as monolayers

Growth Mode:

Adherent

Doubling Time:

ca. 50 hours range 30-72 hours

DNA Profile:

Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12/12,12
D7S820: 8,9
THO1: 6
TPOX: 9,12

Culture Medium:

MEM + 10% FBS+ 1% Non Essential Amino Acids NEAA + 1mM Sodium Pyruvate NaP+ 10 µg/ml human insulininsulinoptional
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium:

90% FBS+10%DMSO

Photo:

Antigen Expression:

Blood Type O; Rh+

Receptor Expression:

Estrogen receptor, expressed

Genes Expressed:

Insulin-like growth factor binding proteins IGFBP BP-2; BP-4; BP-5

Immunology:

cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -

Molec. Genetics:

Expression of fusion gene BCAS4-BCAS3 confirmed by RT-PCR

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, MLV -, SMRV -

Comments:

The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. The cells express the WNT7B oncogene.
Growth of MCF7 cells is inhibited by tumor necrosis factor alpha TNF alpha.