CRL-1646 FO 小鼠骨髓瘤細胞

ATCC® Number:  CRL-1646™      Price:  $355.00 
Designations:  FO 
Depositors:   SF de St. Groth 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: suspension
Organism: Mus musculus （mouse） 
Morphology: lymphoblast

 
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphoblast;
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Antigen Expression: H-2d
Comments: The line was line created by self fusing Sp2/0 myeloma cells using Sendai virus.
The cells are resistant to 8-azaguanine and do not produce immunoglobulin.
The line can be used a fusion partner for making B cell hybridomas.
Tested and found negative for ectromelia virus （mousepox）.
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Subculturing:  Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 to 1 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation:  culture medium 95%; DMSO, 5%
Doubling Time:  8 to 10 hrs 
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1092: de St.Groth SF, Scheidegger D. Production of monoclonal antibodies: strategy and tactics. J. Immunol. Methods 35: 1-21, 1980. PubMed: 7009747
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