5-甲基胞苷-5-三磷酸鹽是TRILINK公司新產品，用于干細胞研究用,5-methyl-ctp
Synonyms]
5-methyl-dCTP
5-methyldeoxycytidine triphosphate
CPD-1094
5-methyl deoxycytidine-5'-triphosphate
5-methyl-2'-deoxycytidine-5'-triphosphate
cytidine 5'-（tetrahydrogen triphosphate）, 2'-deoxy-5-methyl-
[[[5-[（4-amino-5-methyl-2-oxo-1H-pyrimidin-1-yl）]-3-hydroxy-tetrahydrofuran-2-yl]methoxy-hydroxy-phosphinoyl]oxy-hydroxy-phosphinoyl]oxyphosphonic acid
[Structure]
[ Properties Computed from Structure]
| Molecular Weight | 481.183503 [g/mol] |
| Molecular Formula | C10H18N3O13P3 |
| XLogP | -5.9 |
| H-Bond Donor | 6 |
| H-Bond Acceptor | 14 |
| Rotatable Bond Count | 8 |
| Tautomer Count | 3 |
| Exact Mass | 481.005247 |
| MonoIsotopic Mass | 481.005247 |
| Topological Polar Surface Area | 248 |
| Heavy Atom Count | 29 |
| Formal Charge | 0 |
| Complexity | 868 |
| Isotope Atom Count | 0 |
| Defined Atom StereoCenter Count | 0 |
| Undefined Atom StereoCenter Count | 3 |
| Defined Bond StereoCenter Count | 0 |
| Undefined Bond StereoCenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Description 5-Methyl-dCTP is widely used for construction of cDNA libraries | Incorporation of 5-Methyl-dCTP M-MuLV Reverse Transcriptase Klenow Fragment of DNA Polymerase I Sequenase DNA Polymerase （Taq Polymerase, Vent） * Incorporation of Hg-dCTP DNA Polymerase I References to 5-Methyl-dCTP Lefaucheur et al. （1998） Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs. J. Anim. Sci. 76:1584. Nelson et al. （1993） Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios. Nucl. Acids Res. 21 （3）:681. Asamizu et al. （1999） A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Research6:369. * Wong et al. （1991） PCR with 5-methyl-dCTP replacing dCTP. Nucl. Acids Res. 19 （5）:1081. Reference to Hg-dCTP Banfalvi et al. （1995） Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases. DNA Cell Biol. 14 （5）:445. |
| N-1014-1 | 5-Methylcytidine-5'-TP | 1umole | 1317.5 |
| N-1014-10 | 5-Methylcytidine-5'-TP | 10umoles | 10625 |
| N-1014-5 | 5-Methylcytidine-5'-TP | 5umoles | 6205 |
| Reference（s） | ||||
| Kariko K, Muramatsu H, Ludwig J, Weissman D. Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein encoding mRNA. （2011） Nucleic Acids Research. | ||||
| Kormann M, Hasenpusch G, Aneja M, et al. Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. （2011） Nature Biotechnology 29:154–157. | ||||
| Anderson, B., Muramatsu, H., Nallagatla, S.R., Bevilacqua, P.C., Sansing, L.H., Weissman, D. & Kariko, K. Incorporation of pseudouridine into mRNA enhances translation by dimishing PKR activation （2010） Nucleic Acids Research, 38（17）: 5884-5892. | ||||
| Warren et al., Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA, Cell Stem Cell （2010）, doi:10.1016/j.stem.2010.08.012. | ||||
| Kariko K, Muramatsu H, Welsh F, et al. Incorporation of Pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. （2008） Molecular Therapy （16）11: 1833-1840. | ||||
| Kariko, K., Buckstein, M., Ni, H. & Weissman, D. Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modifiation and the Evolutionary Origin of RNA （2005）. Immunity, 23（2）, 165-175. | ||||
| Lefmann M, et al. Novel Mass Spectrometry-based tool for genotypic identification of mycobacteria. （2004） Journal of Clinical Microbiology, 42（1）: 339-346. | ||||
| Hartmer R, Storm N, Boecker S, Rodi CP, Hillenkamp F, Jurinke C, van den Boom D. RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis. （2003） Nucleic Acids Res., 31（9）: e47. | ||||
| Nguyen A, Zhao C, Dorris D, Mazumder A. Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratios. （2002） BMC Biotechnology, 2（1）: 14. | ||||
| Van Rompay AR, Norda A, Linden K, Johansson M, Karlsson A. Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases. （2001） Mol Pharmacol., 59（5）: 1181-6. | ||||
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