聯系電話
翌圣生物科技（上海）股份有限公司
初級會員·14年
具體成交價以合同協議為準
- 聯系人：
- 曹女士
- 電話：
- 400-6111-883
- 手機：
- 售后：
- 4006-111-883
- 傳真：
- 86-21-34615995
- 地址：
- 上海市浦東新區天雄路166弄1號3樓
- 網址：
- www.yeasen.com
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產品簡介
| 供貨周期 | 現貨 | 應用領域 | 生物產業 |
|---|
產品介紹
產品介紹
Tissue non-specific alkaline phosphatase (TNAP) is an isoenzyme of alkaline phosphatase encoded by the ALPL gene in the human body. TNAP is mainly present in tissues such as bone, liver, and kidneys. It plays a crucial role in bone by participating in the metabolism of phosphates and the mineralization process of the skeleton. Additionally, TNAP also plays a role in fetal development and growth. In clinical practice, the activity level of TNAP is also used as a diagnostic marker for bone diseases and liver diseases. Tissue non-specific alkaline phosphatase (TNAP) is found in many different organs, but abundant in the skeletal, hepatic and renal tissues. TNAP is a homodimeric protein and in its structure, in addition to one phosphate anion, each monomer is composed of three metallic ions (one Mg2+ and two Zn2+ cations). Each subunit contains an extended central core β-beach with α-helices, while an extended N-terminal α-helix has a “crown domain". The crown domain can be characterised as a loose interfacial loop with amino acid residues involved in stabilizing the binding of non-competitive inhibitors to the enzyme.
產品性質
產品特色
高純度、高活性、低內毒素、高批間一致性
應用案例
SDS-PAGE
Figure 1.On SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Figure 2. Biotinylated Cynomolgus ALPL Protein; His-Avi Tag was immobilized at 1 μg/ml (100 μL/well) on streptavidin precoated. Increasing concentrations of Anti-His mIgG2a Antibody were added.
存儲條件
-85 ~ -65℃保存，收到貨之日起，6個月有效期。避免反復凍融，建議等分分裝為一次實驗使用量，分裝后，-80℃凍存。
