乳膠微球產品信息

Latex microsphere product information
產品描述：

Product Description:
乳膠微球在免疫診斷學方面有著非常廣泛的應用。采用乳膠微球可以從血清、尿和腦脊液中檢測出微量的抗原或者抗體。微量的抗體可以使抗原包被的微球產生明顯的聚集；同樣微量的抗原也可以使抗體包被的微球產生一樣明顯的聚集。

Latex microspheres have a very wide range of applications in immunodiagnosis.Latex microspheres can detect trace amounts of antigens or antibodies in serum,urine, and cerebrospinal fluid. Micro amounts of antibodies can causesignificant aggregation of antigen-coated microspheres; The same trace amountof antigen can also cause similar aggregation of antibody coated microspheres.

另外一種較常見的用途是將其應用在乳膠凝集抑制試驗中。將待測樣品中的抗原與已知抗體作用后，再與相應抗原包被的乳膠微球混合。因沒有游離抗體的存在，乳膠顆粒表面的抗原不能與抗體結合出現凝集現象，即凝集被抑制。

Another common use is to apply it in latex agglutinationinhibition tests. After reacting the antigen in the test sample with knownantibodies, mix it with the corresponding antigen coated latex microspheres.Due to the absence of free antibodies, the antigen on the surface of latexparticles cannot bind to the antibody, resulting in agglutination, which issuppressed.

例如，孕婦尿液中含HCG，用抗HCG與之結合后，再加HCG包被的乳膠，則不出現凝集現象。乳膠微球也常常被應用于細胞吞噬研究，在此應用中常常使用粒徑從小于50nm到3μm之間大小的微球。

For example, if the urine of pregnant women contains HCG, andanti HCG is combined with it, then latex coated with HCG is added, there willbe no agglutination phenomenon. Latex microspheres are often used in cellphagocytosis research, with particle sizes ranging from less than 50nm to 3 μMicrospheres of size between m.

現有羧基/氨基聚苯乙烯微球、羧基/氨基聚甲基丙烯酸甲酯微球和表面空白的聚苯乙烯微球三種微球。其中羧基/氨基聚苯乙烯微球和表面空白的聚苯乙烯微球的材質為聚苯乙烯高聚物，并用二乙烯基苯交聯，形成網狀聚合物結構；以及羧基/氨基聚甲基丙烯酸甲酯微球的材質為聚甲基丙烯酸甲酯。產品為水分散體系，有很好的力學性能、耐溶劑、耐各種pH環境，以及表面富含羧基/氨基和硫酸根，在溶液中可以保持很好的穩定性，可以適用于各種緩沖溶液。微球表面光滑、平整，比表面積大，并具有很好的均一性、單分散性和穩定性。此類羧基/氨基微球可以較容易地將羧基/氨基與蛋白進行共價偶聯，而表面空白的聚苯乙烯微球可以與蛋白通過疏水締合作用進行連接。在免疫比濁檢查、免疫熒光檢測、ELISA增強、乳膠凝集試驗等領域有著廣泛的應用。

There arethree kinds of microsphere: carboxyl/amino polystyrene microsphere,carboxyl/amino polymethyl methacrylate microsphere and polystyrene microspherewith blank surface. The carboxyl/amino polystyrene microspheres and the surfaceblank polystyrene microspheres are made of polystyrene high polymer andcrosslinked with divinylbenzene to form a network polymer structure; And thecarboxyl/amino polymethyl methacrylate microspheres are made of polymethylmethacrylate. The product is a water dispersible system with excellentmechanical properties, solvent resistance, and resistance to various pHenvironments. The surface is rich in carboxyl/amino groups and sulfateradicals, which can maintain good stability in the solution and can be appliedto various buffer solutions. The surface of the microspheres is smooth andflat, with a large specific surface area and good uniformity, monodispersity,ａnd stability. These carboxyl/amino microspheres can easily covalently couplecarboxyl/amino groups with proteins, while surface blank polystyrenemicrospheres can be connected to proteins through hydrophobic association. Ithas wide applications in fields such as immunoturbidimetry, immunofluorescencedetection, ELISA enhancement, latex agglutination test, etc.

產品優勢：
?       產品多樣化：我們可以提供60nm~700nm之間各種粒徑的微球；
?       良好的穩定性：常溫放置可達24個月。
?       高度的均一性：PDI＜0.050；
?       表面羧基含量高：羧基含量在200μmol/g~1200μmol/g
?       獨特的制備工藝：羧基牢固，不易脫落；
?       產品中無聚丙烯酸類含羧基的游離聚合物存在；
?       微球的強度和韌性佳：在應用中不易被機械破壞；

Product advantages:

Product diversification: We can provide microspheres withvarious particle sizes between 60nm and 700nm;

Good stability: can be stored at room temperature for up to 24months.

? Heightuniformity: PDI ＜ 0.050;

High surface carboxyl content: carboxyl content at 200 μMol/g~1200 μ Mol/g

Unique preparation process: The carboxyl group is firm and noteasy to peel off;

There are no free polymers containing carboxyl groups such aspolyacrylic acid in the product;

The strength and toughness of microspheres are excellent: theyare not easily mechanically damaged in applications;

產品用途

1).免疫比濁試劑，用作抗體、抗原載體.

2).作為酶制劑的普適性支撐材料，用作固定化酶載體

3).ELISA試劑盒開發，用作二抗與HRP載體

4).粒徑標準品.

Product Usage

1) Immunoturbidimetric reagent, used as an antibody and antigencarrier

2) As a universal support material for enzyme preparation, as acarrier for Immobilized enzyme

3) Development of an ELISA kit as a carrier for secondaryantibodies and HRP

4) Particle size standard

應用領域

乳膠微球可以作為診斷試劑的原材料用于抗體／抗原分子的負載，在診斷試劑的應用中起到信號增強的作用。通過載體的放大作用，診斷試劑產品的檢測靈敏度可以提高幾個數岱級。主要可以應用在以下幾方面

application area

Latex microspheres can be used as raw materials for diagnosticreagents to load antibody/antigen molecules, playing a role in signalenhancement in the application of diagnostic reagents. Through theamplification effect of the carrier, the detection sensitivity of diagnosticreagent products can be improved by several orders of magnitude. Mainlyapplicable in the following aspects

1免疫熒光檢測

免疫熒光檢測的原理是先將抗體固載于微球表面，負載于微球表面的抗體與抗原特異性結合后，再加入熒光標記的第二抗體與抗原結合，使微球－抗體－抗原－第二抗體－熒光標記復合物具有穩定的熒光信號，經過洗滌后，用儀器檢測熒光信號。熒光信號的強度與披測抗原的濃度有較強的相關性。

目前市場上采用免疫熒光法的檢測試劑產品有： (1)甲胎蛋白定撮檢測試劑盒（免疫熒光法）： (2) 癌胚抗原定量測定試劑盒（時間分辨免疫熒光法）： (3) 乙型肝炎病毒表面抗原定岱測定試劑盒（時間分辨免疫熒光分析法）: (4) 支原體檢測試劑盒（免疫熒光法）： (5) 抗核抗體 (ANA)間接免疫熒光法檢測試劑盒：等等。

1. Immunofluorescence detection

The principle of immunofluorescence detection is to firstimmobilize antibodies on the surface of microspheres, and then add a secondantibody labeled with fluorescence to bind to the antigen. This results in astable fluorescence signal of the microsphere antibody antigen second antibodyfluorescence labeled complex. After washing, the fluorescence signal isdetected using an instrument. There is a strong correlation between theintensity of fluorescence signals and the concentration of detected antigens.

At present, immunofluorescence detection reagent products in themarket are: (1) AFP assay kit (immunofluorescence method); (2) Carcinoembryonicantigen quantitative assay kit (time-resolved immunofluorescence method); (3)hepatitis B virus surface antigen assay kit (time-resolved immunofluorescencemethod); (4) Mycoplasma detection kit (immunofluorescence method); (5)Antinuclear antibody (ANA) Indirect immunofluorescence assay kit, etc.

2膠乳顆粒增強免疫比濁法

膠孔顆粒增強免疫比濁法 (PETIA) 分為兩種。一種是散射比濁檢測法：另一種是透射比濁檢測法。這兩種方法的基本原理都是在高分子膠孔微球的表面交聯抗體或抗原，當交聯有抗體或抗原的微球與抗原或抗體結合后，在短時間內會迅速聚集在一起，改變了反應液的散光性能或透光性能。而且，反應液散光性能或透光性能（即吸光度）的改變與被測抗原或抗體的濃度有較強的相關性，在一定范圍內可以反映被測物的濃度

目前市場上采用免疫比濁法的膠乳免疫比濁試劑產品有： (1) 腦肋膚檢測試劑： (2) a1-微球蛋白檢測試劑： (3)糖化血紅蛋白檢測試劑: (4)脂蛋白a檢測試劑：

(5) 高敏C-反應蛋白檢測試劑； (6) C-反應蛋白檢測試劑： (7) 抗鏈球菌溶血素"O"檢測試劑： (8)結合蛋白檢測試劑： (9)：< 10) 132微球蛋白檢洌試劑， (11) ： < 12) D-二聚體檢測試劑： < 13) CK-MB 檢測試劑: < 14) 胱抑素C 檢測試劑： (15) 肌紅蛋白檢測試劑； (16) 甲胎蛋白檢測試劑： (17) 肌鈣蛋白檢測試劑：(18) 心型脂肪酸結合蛋白檢測試劑；等等。

2 latex particles enhanced immunoturbidimetry

There are two methods for enhancing immune turbidimetry usingcolloidal particles (PETIA). One is scattering turbidimetric detection, and theother is transmission turbidimetric detection. The basic principle of both methodsis to cross link antibodies or antigens on the surface of polymer gel porousmicrospheres. When the cross linked microspheres with antibodies or antigensare combined with antigens or antibodies, they will quickly gather together ina short time, changing the dispersion or transmittance of the reactionsolution. Moreover, there is a strong correlation between the changes in theastigmatism or transmittance (i.e. absorbance) of the reaction solution and theconcentration of the tested antigen or antibody, which can reflect theconcentration of the tested substance within a certain range

At present, latex immunoturbidimetric reagent products on themarket using immunoturbidimetry include: (1) brain rib skin detection reagent:(2) a1 microglobulin detection reagent: (3) Glycated hemoglobin detectionreagent: (4) lipoprotein a detection reagent:

(5) High sensitivity C-reactive protein detection reagent; (6)C-reactive protein test reagent: (7) anti Streptolysin "O" testreagent: (8) Retinol binding protein test reagent: (9) Rheumatoid factor testreagent:<10) 132 microglobulin test reagent, (11) neutrophil Gelatinaserelated lipid carrier protein test reagent:<12) D-dimer test reagent:<13)CK-MB test reagent:<14) Cystatin C test reagent: (15) Myoglobin testreagent; (16) Alpha fetoprotein test reagent: (17) Troponin test reagent: (18)heart type fatty acid binding protein test reagent; Wait a minute.

3 ELISA反應的增強

此類應用是基千ELISA反應的原理，使用粒徑范圍在 60-200nm的乳膠微球，用酶標儀進行檢測。區別在于傳統的ELISA反應采用了酶標抗體，而此應用是將酶和抗體一起偶聯到微球的表面，使一個微球表面連接有多個酶分子和抗體分子，將偶聯有大晝酶分子和抗體分子的微球代替傳統的一個酶分子標記一個抗體的酶標抗體對ELISA反應起到一個增強的作用。

Enhanced ELISA response

This type of application is based on the principle of ELISAreaction, using latex microspheres with a particle size range of 60-200nm fordetection using an enzyme-linked immunosorbent assay (ELISA). The difference isthat traditional ELISA reactions use enzyme-linked antibodies, while thisapplication couples enzymes and antibodies to the surface of microspheres,connecting multiple enzyme molecules and antibody molecules on the surface of amicrosphere. Replacing the traditional enzyme-linked antibodies labeled withone enzyme molecule and one antibody, microspheres coupled with daytime enzymemolecules and antibody molecules enhance the ELISA reaction.

4乳膠凝集法

乳膠凝集法是以乳膠顆粒作為載體的一種間接；肚集試驗。即吸附或偶聯可溶性抗原或抗體千其表面，特異性抗體或抗原與之結合后，使該乳膠顆粒發生清晰凝集。凝集反應在玻瑤板上進行，結果在良好的光照下用肉眼觀察，常用千類風濕因子檢查等方面。

4 latex agglutination method

Latex agglutination method is an indirect method using latexparticles as carriers; Duji test. Namely, by adsorbing or coupling solubleantigens or antibodies onto their surfaces, specific antibodies or antigensbind to them, resulting in clear agglutination of the latex particles. Theagglutination reaction was carried out on a glass plate, and the results wereobserved with naked eyes under good light, and commonly used in thousands ofRheumatoid factor tests

羧基聚苯乙烯乳膠微球產品目錄

以下為部分種類微球的馬爾文激光粒度儀表征數據

Thefollowing are the characteristic data of some types of microspheres usingMarvin laser particle size instruments

同時我們對比了三種國外進口產品的馬爾文激光粒度儀表征參數

At thesame time, we compared the characteristic parameters of Marvin laser particlesize instruments for three imported products from abroad