中文摘要：

腱?骨損傷患者因天然結構破壞導致運動功能受限，阻礙損傷恢復。傳統生物材料側重于增強肌腱 / 骨的再生能力以重建天然結構，但由于忽視免疫微環境且缺乏多組織再生功能，往往難以獲得理想的修復效果。本研究將硅酸錳（MS）納米顆粒與肌腱 / 骨相關細胞復合，制備出可用于腱?骨一體化再生的免疫調節型多細胞支架。

值得注意的是，通過整合仿生細胞排布與硅酸錳納米顆粒，該多細胞支架展現出多重生物活性。此外，硅酸錳納米顆?？赏ㄟ^調控巨噬細胞，促進支架內多細胞的特異性分化；該效應主要源于錳離子誘導巨噬細胞分泌前列腺素 E2（PGE2）。多項動物實驗結果證實，該支架能夠在腱?骨界面實現免疫調節、一體化再生及運動功能恢復。

綜上，基于無機生物材料構建的多細胞支架，為軟 / 硬組織界面的免疫調控與一體化再生提供了創新思路。

英文摘要：

Limited motor activity due to the loss of natural structure impedes recovery in patients suffering from tendon-to-bone injury. Conventional biomaterials focus on strengthening the regenerative ability of tendons/bones to restore natural structure. However, owing to ignoring the immune environment and lack of multi-tissue regenerative function, satisfactory outcomes remain elusive. Here, combined manganese silicate (MS) nanoparticles with tendon/bone-related cells, the immunomodulatory multicellular scaffolds were fabricated for integrated regeneration of tendon-to-bone. Notably, by integrating biomimetic cellular distribution and MS nanoparticles, the multicellular scaffolds exhibited diverse bioactivities. Moreover, MS nanoparticles enhanced the specific differentiation of multicellular scaffolds via regulating macrophages, which was mainly attributed to the secretion of PGE2 in macrophages induced by Mn ions. Furthermore, three animal results indicated that the scaffolds achieved immunomodulation, integrated regeneration, and function recovery at tendon-to-bone interfaces. Thus, the multicellular scaffolds based on inorganic biomaterials offer an innovative concept for immunomodulation and integrated regeneration of soft/hard tissue interfaces.

論文信息：

論文題目：Immunomodulatory multicellular scaffolds for tendon-to-bone regeneration

期刊名稱：Science Advances

時間期卷：Vol 10, Issue 10(2024)

在線時間：2024年3月8日

DOI: 10.1126/sciadv.adk6610

產品信息：

貨號：CP-005-005

規格：5ml+5ml

品牌：Liposoma

產地：荷蘭

名稱：Clodronate Liposomes&Control Liposomes

辦事處：靶點科技

Clodronate Liposomes氯膦酸鹽脂質體在肌腱 / 骨相關細胞復合模型種清除巨噬細胞。荷蘭Liposoma巨噬細胞清除劑ClodronateLiposomes見刊于Science Advances：用于腱?骨再生的免疫調節型多細胞支架。

Liposoma巨噬細胞清除劑Clodronate Liposomes氯膦酸二鈉脂質體清除巨噬細胞的材料和方法：

Depletion of AMs

To investigate the effect of macrophage depletion on enhanced regenerative outcomes induced by the scaffolds containing MS nanoparticles, the macrophage depletion model of Sprague-Dawley rats was created according to a previous report (57). Clodronate liposomes (LIPOSOMA, Netherlands) were applied to deplete macrophages. First, the Sprague-Dawley rats (male, 6 weeks old) were randomly divided into two groups: one group was treated with liposome-encapsulated PBS (GelMA-cells-MS+PBS) and another group was treated with clodronate liposomes (GelMA-cells-MS+clodronate). All of the rats received injections on days 1 and 3 before the implantation of scaffolds. After being shaved, the backs of the rats were sterilized with iodine solution. Then, a subcutaneous cavity was created on the side of the back, enabling the insertion of a scaffold. The skin incision was closed by 4-0 Ethibond sutures. Subsequently, sterile liposomal clodronate or liposome-encapsulated PBS (50 mg/kg) was locally injected in the cavity every 3 days from the first postoperative day. After 2 weeks of operation, the rats were euthanized and the scaffolds were collected. When analyzing the above results, the researchers were blinded to the grouping of animals.

巨噬細胞清除材料和方法文獻截圖：用于腱?骨再生的免疫調節型多細胞支架