中文摘要：

瘧原蟲可抑制宿主免疫應答以利于自身存活，但其背后的分子機制尚不明確。本研究發現，紅內期瘧原蟲可顯著誘導 CD4?Foxp3?CD25?調節性 T 細胞分泌可溶性纖維蛋白原樣蛋白 2（sFGL2），該蛋白會大幅加重瘧原蟲感染程度。

英文摘要：

Malaria parasites suppress host immune responses to facilitate their survival, but the underlying mechanism remains elusive. Here, we found that blood-stage malaria parasites predominantly induced CD4+Foxp3+CD25+ regulatory T cells to release soluble fibrinogen-like protein 2 (sFGL2), which substantially enhanced the infection. This was attributed to the capacity of sFGL2 to inhibit macrophages from releasing monocyte chemoattractant protein-1 (MCP-1) and to sequentially reduce the recruitment of natural killer/natural killer T cells to the spleen and the production of interferon-γ. sFGL2 inhibited c-Jun N-terminal kinase phosphorylation in the Toll-like receptor 2 signaling pathway of macrophages dependent on FcγRIIB receptor to release MCP-1. Notably, sFGL2 were markedly elevated in the sera of patients with malaria, and recombinant FGL2 substantially suppressed Plasmodium falciparum from inducing macrophages to release MCP-1. Therefore, we highlight a previously unrecognized immune suppression strategy of malaria parasites and uncover the fundamental mechanism of sFGL2 to suppress host innate immune responses.

論文信息：

論文題目：Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2

期刊名稱：Science Advances

時間期卷：Vol 6, Issue9(2020)

在線時間：2020年2月26日

DOI: 10.1126/sciadv.aay9269

產品信息：

貨號：CP-005-005

規格：5ml+5ml

品牌：Liposoma

產地：荷蘭

名稱：Clodronate Liposomes&Control Liposomes

辦事處：靶點科技

Clodronate Liposomes氯膦酸鹽脂質體靜脈注射，清除瘧原蟲感染模型巨噬細胞。荷蘭Liposoma巨噬細胞清除劑ClodronateLiposomes見刊于Science Advances：紅內期瘧原蟲通過誘導可溶性纖維蛋白原樣蛋白 2（sFGL2）調控宿主固有免疫應答.

Liposoma巨噬細胞清除劑Clodronate Liposomes氯膦酸二鈉脂質體清除巨噬細胞的材料和方法：

In vivo macrophage depletion

For the depletion assay, 200 μg of the corresponding isotype control IgG, anti–IFN-γ mAb (clone XMG1.2, Bio X Cell), or anti–MCP-1 mAb (clone 2H5, Bio X Cell) was injected intraperitoneally into P. chabaudi–infected mice at ?1, 0, 1, 3, and 5 days after infection. Depletion of NK/NKT cells was accomplished by intraperitoneal injection of 200 μg of anti-NK1.1 mAb (clone PK136, Bio X Cell) at ?1, 0, 1, 3, and 5 days after infection. Depletion of Tregs was accomplished by intraperitoneal injection of 400 μg of anti-CD25 mAb (clone PC61.5, Bio X Cell) at ?1, 0, 1, 3, 5, and 7 days after infection. Macrophage depletion in FGL2?/? mice was accomplished by intravenous injection of 200 μl of clodronate liposomes (Liposoma BV) on days ?1, 2, and 5 of malaria parasite infection and with the injection of control liposomes (PBS) as a control. For rescue experiments, FGL2?/? mice received 25 μg of rFGL2 proteins (R&D Systems) by intravenous injection from the first day of infection until 5 days after infection.

巨噬細胞清除材料和方法文獻截圖：紅內期瘧原蟲通過誘導可溶性纖維蛋白原樣蛋白 2（sFGL2）調控宿主固有免疫應答。