Microplates for fluorescence-based assays are black-walled, clear-bottom microplates that minimize well-to-well crosstalk and autofluorescence. These 96- and 384-well microplates are compatible with standard fluorescence microplate readers and optimized for fluorescence-based applications including nucleic acid quantitation (e.g., Quant-iT assays, including PicoGreen, RiboGreen, and OliGreen).