Product nameAnti-ATP5O [4C11C10D12] antibodySee all ATP5O primary antibodies ...
Description
Mouse monoclonal [4C11C10D12] to ATP5O
Tested applicationsICC/IF, WB, Flow Cyt, IHC-P more details
Species reactivity
Reacts with: Cow, Human, African Green Monkey
Immunogen
Recombinant mature Human ATP5O.
Positive control
- Isolated mitochondria from Human heart and Bovine heart, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), Human colon tissue, HL-60 cells

Properties

FormLiquid
Storage instructionsStore at +4°C. Do not freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HBS
Concentration 100 µg at 1 mg/ml
PurityIgG fraction
Purification notesNear homogeneity as judged by SDS-PAGE. ab110276 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality Monoclonal
Clone number4C11C10D12
IsotypeIgG1
Light chain typekappa
Research Areas
- Tags & Cell Markers
- Subcellular Markers
- Organelles
- Mitochondria
- Signal Transduction
- Metabolism
- Mitochondrial
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Mitochondrial markers
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Oxidative phosphorylation
- Complex V
- Metabolism
- Types of disease
- Cancer

Applications

Our Abpromise guarantee covers the use of ab110276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Notes
ICC/IF	ICC/IF: Use a concentration of 0.5 - 1 µg/ml. heat-induced antigen-retrieval improves signal
WB	WB: Use a concentration of 0.5 µg/ml. Predicted molecular weight: 23 kDa.
Flow Cyt	Flow Cyt: Use a concentration of 1 µg/ml.
IHC-P	IHC-P: 1/500.

Target

FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
Sequence similaritiesBelongs to the ATPase delta chain family.
Cellular localizationMitochondrion. Mitochondrion inner membrane.
Information by UniProt
Database links
- Entrez Gene: ATP5O Cow
- Entrez Gene: 539 Human
- Omim: 600828 Human
- SwissProt: P13621 Cow
- SwissProt: P48047 Human
- Unigene: 49172 Cow
- Unigene: 409140 Human
Alternative names
- ATP synthase O subunit mitochondrial precursor antibody
- ATP synthase subunit O antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody

Anti-ATP5O [4C11C10D12] antibody images

Western blot - ATP5O antibody [4C11C10D12] (ab110276)
All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 0.5 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Predicted band size : 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276)
Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp.
Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276)
Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
Flow Cytometry - ATP5O antibody [4C11C10D12] (ab110276)
HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)
All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 1 µg/ml
Lane 1 : HDFn (human) cell lysates at 20 µg
Lane 2 : COS7 (monkey) cell lysates at 20 µg
Lane 3 : H4IIE (rat) cell lysates at 20 µg
Lane 4 : MEF (mouse) cell lysates at 20 µg
Lane 5 : bovine heart mitochondria lysates at 5 µg
Predicted band size : 23 kDa
Secondary antibody: Goat-Anti-Mouse-IR800 1:4000

公司介紹：

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