Lonza AMAXA Lonza Nucleofector 2B /II 細胞核轉染儀

單孔細胞核轉染儀

Ÿ   優化的電穿孔技術

Ÿ   獨特的細胞特異性電轉染程序

Ÿ   程序的不斷更新升級

Lonza(原Amaxa)公司的Nucleofection®技術在傳統的電穿孔技術基礎上取得了突破性進展，通過調整優化電轉參數（內存轉染程序），結合細胞特異性的轉染試劑直接把外源基因導入原代細胞和傳代細胞的細胞漿和細胞核中。在保證高效轉染的同時，也提高了細胞的存活率；

世界上直接把外源基因導入細胞核的創新型技術！

世界上能高效轉染原代細胞、懸浮細胞的非病毒轉染技術！

世界上能在數小時內完成從實驗操作到結果分析全過程的轉染技術！

共享細胞優化轉染程序數據庫；

高效轉染DNA、RNA、peptide等各種底物；

已發表超過5000篇文獻（/nucleofection-citations ）。

的轉染技術，它綜合應用傳統的電穿孔技術及細胞特異性的細胞核轉染液，通過調整優化電轉參數（內存程序），直接把外源基因導入原代細胞和傳代細胞核中。針對免疫細胞、神經細胞、干細胞等幾乎所有難轉染的細胞系或原代細胞或懸浮細胞。
 

Transfection is an essential tool for numerous applications, including studies of gene expression. However, transfection of cells with common methods such as calcium phosphate, DEAE-dextran, liposome-mediated transfer, electroporation or viral vectors is limited because of low transfection efficiency and the extended time required for analysis of gene expression. Amaxa Biosystems has a new electroporation-based transfection method that enables the DNA to directly enter the nucleus while cells are stabilized in an electric field by treatment with a specific solution. The method is called “Nucleofection” and the electoporator system is called Nucleofector II. The system has built-in optimized programs for most common cell types.

The transfection procedure is simple and straightforward with these basic steps: (1) Preparing cells to be transfected with the nucleofection solution appropriate for the cell type, (2) Adding the nucleic acid to the prepared cells and transferring the mixture to a cuvette (provided in the kit), (3) Placing the cuvette in the system, electroporating cells with the optimized program for the cell type, (4) Harvesting cells from the cuvette and dispersing on to media contained in a dish. The whole process can be done in less than 30 minutes.

The good part about the system is that the method is easy, simple (some optimization required with regard to the appropriate amount of DNA and the type of program used for each cell type), sensitive, requires minimal expertise and no prior plating of cells to be transfected is needed. This in contrast to the alternative methods which are limited by the need to plate cells at a particular density 24-48 hours prior to transfection. In addition, these methods are labor and time intensive. Amaxa has nucleofection kits with optimized protocols available for most common cell types. As nucleic acids are delivered straight into the nucleus, nucleofected cells can be ready for analysis after 2–6 hrs of transfection.

 

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