中文摘要：

通過化學重編程技術將人體體細胞誘導為巨核細胞，是研發新型替代血小板來源的優質策略。本團隊在前期紅細胞向巨核細胞化學重編程實驗方案的基礎上，建立了一套穩定高效的誘導體系，成功利用來源更為豐富的人臍血CD3+T細胞誘導產生誘導型巨核細胞。該體系采用包含重編程增效劑AZD4205的五小分子組合配方，可清除T細胞固有細胞特征，助力細胞向巨核細胞譜系發生命運轉換。

英文摘要：

The generation of megakaryocytes (MKs) from human somatic cells through chemical reprogramming represents a promising strategy for developing alternative platelet sources. Building on our prior chemical reprogramming protocol for converting erythroblasts to MKs, we established a robust method that successfully generated induced MKs (iMKs) from human cord blood–derived CD3+ T cells, which is a more abundant source. This method used a five–small molecule cocktail containing a reprogramming booster, AZD4205, to promote erasure of T cell identity and facilitate fate transition toward MKs. T cell–derived iMKs exhibited characteristic MK cellular and molecular signatures, demonstrating the capacity to produce proplatelets and release functional platelets. Single-cell RNA sequencing further revealed that iMKs were heterogeneous with distinct functional profiles, including cycling, immune, and thrombopoiesis-biased MKs. Our findings highlight an optimized chemical reprogramming strategy that enables efficient conversion of T cells to MKs, providing a practical and convenient approach to generating clinically relevant MKs and platelets.

論文信息：

論文題目：Efficient chemical reprogramming of human T cells into functional megakaryocytes and platelets

期刊名稱：Science Advances

時間期卷：Vol 12, Issue29(2026)

在線時間：2026年7月15日

DOI: 10.1126/sciadv.aeb31

產品信息：

貨號：CP-005-005

規格：5ml+5ml

品牌：Liposoma

產地：荷蘭

名稱：Clodronate Liposomes&Control Liposomes

辦事處：靶點科技

Clodronate Liposomes氯膦酸鹽脂質體清除經Day-7輻照的免疫缺陷小鼠體內巨噬細胞。荷蘭Liposoma巨噬細胞清除劑ClodronateLiposomes見刊于Science Advances：高效化學重編程人T細胞為功能性巨核細胞與血小板。

Liposoma巨噬細胞清除劑Clodronate Liposomes氯膦酸二鈉脂質體清除巨噬細胞的材料和方法：

In vivo macrophage depletion

On day ?7, 8- to 12-week-old male NVSG mice (Viewsolid Biotech Co. Ltd., Beijing, China) were irradiated (2.5 Gy) to induce thrombocytopenia. On day ?1, the mice were depleted of macrophages by treatment with 0.04 mg of clodronate liposomes (Liposoma Technology, Amsterdam, Netherlands) per gram of mouse weight. iMKs were stained with 5 μM DiD (Thermo Fisher Scientific, V22887) for 20 min at 37°C before washing. PBS (400 μl) containing 1 × 107 cells was injected into mice through the tail vein. Human platelets were monitored by flow cytometry from peripheral blood samples at 1, 3, 6, 9, and 24 hours after transfusion using antibodies specific for human CD41a. The absolute platelet count was determined using a Mindray Bc-2800 Vet Blood Cell Counter (Mindray, Shenzhen, China). The mice were euthanized 24 hours after transfusion, and samples from the spleen, bone marrow, and lungs were collected. All mice were kept under specific pathogen–free conditions, and all procedures were performed according to protocols approved by the Institutional Animal Care and Use Committee at the Institute of Radiation Medicine.

巨噬細胞清除材料和方法文獻截圖：高效化學重編程人T細胞為功能性巨核細胞與血小板